1,2,4-Trimethoxybenzeen

1,2,4-Trimethoxybenzene is an orally active NLRP3 selective inhibitor. 1,2,4-Trimethoxybenzene can markedly suppress Nigericin or ATP (HY-B2176) -induced NLRP3 inflammasome activation, thus decreasing caspase-1 activation and IL-1β secretion. 1,2,4-Trimethoxybenzene specifically inhibits the activation of NLRP3 inflammasome without affecting absent in melanoma 2 (AIM2) inflammasome activation. 1,2,4-Trimethoxybenzene inhibits oligomerization of the apoptosis-associated speck-like protein containing a CARD (ASC) and protein-protein interaction between NLRP3 and ASC, thus blocking NLRP3 inflammasome assembly. 1,2,4-Trimethoxybenzene can be used for the study of experimental autoimmune encephalomyelitis (EAE), multiple sclerosis, and type 2 diabetes[1][2][3].

135-77-3

12352005

H302, H315, H319, H335

Caspase; Interleukin Related; NOD-like Receptor (NLR)

Apoptosis; Immunology/Inflammation

Infection; Metabolic Disease

98.43

COC(C(OC)=C1)=CC=C1OC

C9H12O3

168.19

H302, H315, H319, H335

[1]Pan RY, et al. 1,2,4-Trimethoxybenzene selectively inhibits NLRP3 inflammasome activation and attenuates experimental autoimmune encephalomyelitis. Acta Pharmacol Sin. 2021 Nov;42 (11) :1769-1779. doi: 10.1038/s41401-021-00613-8IF: 8.4 Q1 . Epub 2021 Feb 24. Erratum in: Acta Pharmacol Sin. 2022 Feb;43 (2) :504.|[2]Zhang Y, et al. 1,2,4-Trimethoxybenzene ameliorates depression-like behaviors by inhibiting the activation of NLRP3 inflammasome. Int Immunopharmacol. 2025 Apr 4;151:114361.|[3]Zhong P, et al. 1,2,4-Trimethoxybenzene alleviates cognitive dysfunction in type 2 diabetes model rats by inhibiting NLRP3 inflammasome and restoring gut microbiota disorders. Biochem Pharmacol. 2025 Dec;242 (Pt 4) :117409.

Room Temperature

Store at room temperature 3 years

http://file.medchemexpress.com/batch_PDF/HY-W017087/

Reference compound2

No Development Reported

Caspase 1; IL-1; NLRP3