3T3-L1 Lysaat

3T3-L1 cell line was derived from mouse 3T3 cells that is used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype. 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. These cells are also sensitive to lipogenic and lipolytic hormones and drugs, including epinephrine, isoproterenol, and insulin. 3T3-L1 cells are extensively used to study adipogenesis.

Mouse

WB

2 mg/mL (determined by BCA assay)

3T3-L1, ATGL0021-10 µg, ATGL0021-20 µg, ATGL0021-50 µg, ATGL0021-100 µg, ATGL0021-250 µg, ATGL0021-500 µg, ATGL0021-1 mg, ATGL0021-10, ATGL0021-20, ATGL0021-50, ATGL0021-100, ATGL0021-250, ATGL0021-500, ATGL0021-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : 3T3-L1 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody