4E-BP1 Antilichaam [9E12D9]

4E-BP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP-1/PHAS-I , which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa) . Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while (24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

1978

Q13541

Q13541

Human

Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3) .

9E12D9

Unconjugated

Primary Antibodies

Signal Transduction

Batch dependent

Ascitic fluid containing 0.03% sodium azide.

None

Blue Ice

4E-BP1 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1

EIF4EBP1

Eukaryotic translation initiation factor 4E binding protein 1

Homo sapiens

Pause, A. et al. 1994.Nature. 371:762–767.

Fadden, P. et al. 1997. J. Biol. Chem. 272:10240–10247.

Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I

ELISA, WB

34921508