5-HIAA ELISA [CE]

5-HIAAis quantitatively derivatized by methylation. The subsequent competitiveELISAuses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG

HRP

ELISA

Urine

50 µL

0,4-50 mg/L

0.16 mg/L

ELISA

60 Weeks

https://cdn.gentaur.com/products/22/69ae95b01125b5183b569bd9/ifu/kapl10-1900.pdf

3h

Biogenic Amines and Neurosciences

5HIAA

2 levels