5-Methyl-2'-deoxycytidine (standaard)

5-Methyl-2'-deoxycytidine (5mdC) is an endogenous substrate of DNA methyltransferases (such as mammalian 5-C-MTase) and binds to DNA dependent on the formation of DNA stem-loop structures. 5-Methyl-2'-deoxycytidine guides de novo DNA methylation by acting as a methylation mark and activates the methylation of adjacent CpG sites in single-stranded DNA through cis action. 5-Methyl-2'-deoxycytidine regulates DNA methylation patterns by recruiting methyltransferases to specific chromatin regions, affecting chromatin condensation and gene expression. Its distribution in plant cells is related to cell proliferation and differentiation stages. The methylation level of 5-Methyl-2'-deoxycytidine is low in proliferating cells and high in differentiated cells[1][2][3].

838-07-3

12352005

DNA Methyltransferase; Endogenous Metabolite

Epigenetics; Metabolic Enzyme/Protease

Others

OC[C@@H]1[C@@H](O)C[C@H](N2C(N=C(N)C(C)=C2)=O)O1

C10H15N3O4

241.25

[1]Christman JK, et al. 5-Methyl-2'-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation. Proc Natl Acad Sci U S A. 1995 Aug 1;92 (16) :7347-51.|[2]Testillano PS, et al. The 5-methyl-deoxy-cytidine (5mdC) localization to reveal in situ the dynamics of DNA methylation chromatin pattern in a variety of plant organ and tissue cells during development. Physiol Plant. 2013 Sep;149 (1) :104-13.|[3]Magaña AA, et al. High-performance liquid chromatography determination of 5-methyl-2'-deoxycytidine, 2'-deoxycytidine, and other deoxynucleosides and nucleosides in DNA digests. Anal Biochem. 2008 Mar 15;374 (2) :378-85.

Room temperature

http://file.medchemexpress.com/batch_PDF/HY-W012078R/

Reference Standards