A427 Lysaat

A427 cells are derived from human lung epithelial carcinoma taken from a 52 year-old male in 1973.

Human

WB

2 mg/mL (determined by BCA assay)

A427, ATGL0028-10 µg, ATGL0028-20 µg, ATGL0028-50 µg, ATGL0028-100 µg, ATGL0028-250 µg, ATGL0028-500 µg, ATGL0028-1 mg, ATGL0028-10, ATGL0028-20, ATGL0028-50, ATGL0028-100, ATGL0028-250, ATGL0028-500, ATGL0028-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : A427 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody