A431 Lysaat

A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85 year old female patient. A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the EGFR. As such they are often used as a positive control for EGFR expression.

Human

WB

2 mg/mL (determined by BCA assay)

A431, ATGL0007-10 µg, ATGL0007-20 µg, ATGL0007-50 µg, ATGL0007-100 µg, ATGL0007-250 µg, ATGL0007-500 µg, ATGL0007-1 mg, ATGL0007-10, ATGL0007-20, ATGL0007-50, ATGL0007-100, ATGL0007-250, ATGL0007-500, ATGL0007-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : A431 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody