Aak1 Antilichaam

Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

22848

Q2M2I8

NP_055726

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Signal Transduction

Aak1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1288 - A20 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%)

Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Aak1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

AAK1

AP2 associated kinase 1

Homo sapiens

Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9.

Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep.2003; 4:246-51.

Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol.2002; 156:791-5.

Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell2007; 18:2698-706.

Aak1 Antibody: KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1

ELISA, WB

148277037

Liquid