AARI

AarI is an isoschizomer of PaqCI. Type IIS restriction enzyme which recognizes the sequence CACCTGC and cleaves outside of their recognition sequence, generating 4-base 5' overhangs. Especially, AarI requires two or more sites for digestion. AarI Activator supplied to improve cleavage. It recognizes a 7-basesequence that occurs infrequently in genes, is particularly suitable for Golden Gate assembly.

http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/25526.pdf

• AarI (10 U/μl) : 20 μl • AarI Activator: 20 μl • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 75°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Store at -20°C for 2 years.

PaqCI

CACCTGC (4/8) 5'...C A C C T G C (N) 4↓...3' 3'...G T G G A C G (N) 8↑...5'