AB Antilichaam

AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) . Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.

9131

O95381

O95381

Human

2239

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar AIF expression profile in human and mouse cell lines detected by three independent anti-AIF antibodies that recognize different epitopes, 2267 against central domain, 2239 against N-terminus domain and 2301 against C-terminus domain. AIF proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. Independent Antibody Validation in Cell lines2267 22392301 KD Validation (Figure 4,5) : Anti-AIF antibody specificity was further verified by AIF specific knockdown. AIF signal in H1299 cells and AIF stable cells transfected with AIF siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD Validation Localization Validation (Figure 7) : AIF detected by anti-AIF antibodies is colocalized with Mitotracker (mitochondrion marker) . AIF is localized in mitochondria. Localization Validation Induced Expression Validation (Figure 8) : AIF expression detected by anit-AIF antibodies was up-regulated by Magnolol treatment. Induced Expression Validation

Apoptosis, Cancer

AIF Antibody is Ion exchange chromatography purified.

Cat. No. 1204 - K562 Cell Lysate

1 mg/ml

Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (50%)

AIF Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

AIF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Multiple isoforms of AIF are known to exist.

Human AIF has 6 isoforms, including isoform 1 (613aa, 67kD), isoform 2 (326aa, 36kD), isoform 3 (609aa, 66kD), isoform 4 (324aa, 35kD), isoform 5 (261aa, 28kD), and isoform 6 (237aa, 26kD) . Mouse AIF has one isoform (612aa, 67kD) and Rat AIF also has one isoform (612aa, 67kD) . 2239 can detect only 4 human isoforms (1,3,4,6) .

AIFM1

Cat. No. 1205 - Jurkat Cell Lysate

Cat. No. 17-005 - Jurkat Cell Slide

Cat. No. 17-004 - K-562 Cell Slide

Apoptosis inducing factor mitochondria associated 1

Homo sapiens

Zamzami et al. Nature 1999; 401:127-8.

Susin et al. Nature 1999; 397:441-6

Daugas et al. FASEB J. 2000; 14:729-39.

16729031

16195738

21937913

12939460

17142989

15619633

15131059

15126337

18519664

15297883

16645637

17553685

17980607

16273274

15699578

12140745

16454040

19414401

12140745

AIF Antibody: Apoptosis-inducing factor 1, Programmed cell death protein 8, AIF, PDCD8

ELISA, WB, IF

50400606

Liquid