ABI3 cDNA

ABI3 encodes a member of an adaptor protein family. Members of this family encode proteins containing a homeobox homology domain, proline rich region and Src-homology 3 (SH3) domain, and are components of the Abi/WAVE complex which regulates actin polymerization. The encoded protein inhibits ectopic metastasis of tumor cells as well as cell migration. This may be accomplished through interaction with p21-activated kinase. Alternative splicing results in multiple transcript variants.

NESH, SSH3BP3

17q21.3

Human

PATGen (puc19-derived cloning vector)

ATGGCGGAGCTACAGCAGCTGCAGGAGTTTGAGATCCCCACTGGCCGGGAGGCTCTGAGGGGCAACCACAGTGCCCTGCTGCGGGTCGCTGACTACTGCGAGGACAACTATGTGCAGGCCACAGACAAGCGGAAGGCGCTGGAGGAGACCATGGCCTTCACTACCCAGGCACTGGCCAGCGTGGCCTACCAGGTGGGCAACCTGGCCGGGCACACTCTGCGCATGTTGGACCTGCAGGGGGCCGCCCTGCGGCAGGTGGAAGCCCGTGTAAGCACGCTGGGCCAGATGGTGAACATGCATATGGAGAAGGTGGCCCGAAGGGAGATCGGCACCTTAGCCACTGTCCAGCGGCTGCCCCCCGGCCAGAAGGTCATCGCCCCAGAGAACCTACCCCCTCTCACGCCCTACTGCAGGAGACCCCTCAACTTTGGCTGCCTGGACGACATTGGCCATGGGATCAAGGACCTCAGCACGCAGCTGTCAAGAACAGGCACCCTGTCTCGAAAGAGCATCAAGGCCCCTGCCACACCCGCCTCCGCCACCTTGGGGAGACCACCCCGGATTCCCGAGCCAGTGCACCTGCCGGTGGTGCCCGACGGCAGACTCTCCGCCGCCTCCTCTGCGTCTTCCCTGGCCTCGGCCGGCAGCGCCGAAGGTGTCGGTGGGGCCCCCACGCCCAAGGGGCAGGCAGCACCTCCAGCCCCACCTCTCCCCAGCTCCTTGGACCCACCTCCTCCACCAGCAGCCGTCGAGGTGTTCCAGCGGCCTCCCACGCTGGAGGAGTTGTCCCCACCCCCACCGGACGAAGAGCTGCCCCTGCCACTGGACCTGCCTCCTCCTCCACCCCTGGATGGAGATGAATTGGGGCTGCCTCCACCCCCACCAGGATTTGGGCCTGATGAGCCCAGCTGGGTGCCTGCCTCATACTTGGAGAAAGTGGTGACACTGTACCCATACACCAGCCAGAAGGACAATGAGCTCTCCTTCTCTGAGGGCACTGTCATCTGTGTCACTCGCCGCTACTCCGATGGCTGGTGCGAGGGCGTCAGCTCAGAGGGGACTGGATTCTTCCCTGGGAACTATGTGGAGCCCAGCTGCTGA

ABI3, NESH, SSH3BP3, ABI3, ATGD0281-10 µg, ATGD0281-20 µg, ATGD0281-50 µg, ATGD0281-100 µg, ATGD0281-250 µg, ATGD0281-500 µg, ATGD0281-1 mg, ATGD0281-10, ATGD0281-20, ATGD0281-50, ATGD0281-100, ATGD0281-250, ATGD0281-500, ATGD0281-1

Store the plasmid at -20C.

Lyophilized

Cancer

606363

NP_057512.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_016428.2

1101bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.