ACACB Antilichaam / Acetyl-CoA carboxylase 2

Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

O00763

Human, Mouse, Rat

A peptide from the C-terminal region of human Acetyl-CoA carboxylase 2 protein was used as the immunogen for this ACACB antibody.

WB, IHC-P, FACS

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.05% sodium azide

This ACACB antibody is available for research use only.

After reconstitution, the ACACB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the ACACB antibody should be determined by the researcher.

Cytoplasm (Mitochondria)

IHC staining of FFPE rat testis tissue with ACACB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.