ACK1 (fosfo Tyr284) Antilichaam

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.

10188

Q07912

NP_005772.3

Human, Mouse

ACK1 (phospho Tyr284) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y (p) -V-M) derived from Human ACK1.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

120 kDa

IgG

The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

TNK2

Tyrosine kinase, non-receptor, 2

Homo sapiens

Manser E., Nature 363:364-367 (1993) .

Ota T., Nat. Genet. 36:40-45 (2004) .

Eisenmann K.M., Nat. Cell Biol. 1:507-513 (1999)

ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1

WB, IHC, IF

229462980

Liquid