ACVR2A Antilichaam / Activa receptor type-2A

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P27037

Human

E.coli-derived human ACVR2A recombinant protein (Position: A20-N138) was used as the immunogen for the ACVR2A antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ACVR2A antibody is available for research use only.

After reconstitution, the ACVR2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ACVR2A antibody should be determined by the researcher.

Western blot analysis of ACVR2A using anti-ACVR2A antibody. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of ACVR2A is ~58 kDa.