Aequorea victoria GFP antilichaam

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Green fluorescent protein

GFP

Aequorea victoria

Recombinant GFP (1-238aa) purified from E. coli

AT2G5

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

GFP, ATGA0148-10 µg, ATGA0148-20 µg, ATGA0148-50 µg, ATGA0148-100 µg, ATGA0148-250 µg, ATGA0148-500 µg, ATGA0148-1 mg, ATGA0148-10, ATGA0148-20, ATGA0148-50, ATGA0148-100, ATGA0148-250, ATGA0148-500, ATGA0148-1

Phillips G (2001) FEMS Microbiol Lett 204 (1) :9-18.; ; Tsien R (1998) Annu Rev Biochem 67:509-544.; ; Prasher, et al., (1992) Gene 111 (2) :229-233.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Fusion Tag & Detection

P42212

The recombinant proteins were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GFP protein (20ng) ; ; Lane 2.: Recombinant human GFP protein (40ng) ; ; Lane 3.: Recombinant human GFP protein (80ng) The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: GFP transfected 293T cell lysate {ATGA0148-WB2.jpg}