AES cDNA

Amino-terminal enhancer of split, also known AES, belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. This protein is expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle. In addition, AES can repress NFkB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events.

AES-1, AES-2, ESP1, GRG, Grg-5, GRG5, TLE5

19p13.3

Human

PATGen (puc19-derived cloning vector)

ATGATGTTTC CACAAAGCAG GCATTCGGGC TCCTCGCACC TACCCCAGCA ACTCAAATTC ACCACCTCGG ACTCCTGCGA CCGCATCAAA GACGAATTTC AGCTACTGCA AGCTCAGTAC CACAGCCTCA AGCTCGAATG TGACAAGTTG GCCAGTGAGA AGTCAGAGAT GCAGCGTCAC TATGTGATGT ACTACGAGAT GTCCTACGGC TTGAACATCG AGATGCACAA ACAGGCTGAG ATCGTCAAAA GGCTGAACGG GATTTGTGCC CAGGTCCTGC CCTACCTCTC CCAAGAGCAC CAGCAGCAGG TCTTGGGAGC CATTGAGAGG GCCAAGCAGG TCACCGCTCC CGAGCTGAAC TCTATCATCC GACAGCAGCT CCAAGCCCAC CAGCTGTCCC AGCTGCAGGC CCTGGCCCTG CCCTTGACCC CACTACCCGT GGGGCTGCAG CCGCCTTCGC TGCCGGCGGT CAGCGCAGGC ACCGGCCTCC TCTCGCTGTC CGCGCTGGGT TCCCAGGCCC ACCTCTCCAA GGAAGACAAG AACGGGCACG ATGGTGACAC CCACCAGGAG GATGATGGCG AGAAGTCGGA TTAG

AES, AES-1, AES-2, ESP1, GRG, Grg-5, GRG5, TLE5, AES, ATGD0043-10 µg, ATGD0043-20 µg, ATGD0043-50 µg, ATGD0043-100 µg, ATGD0043-250 µg, ATGD0043-500 µg, ATGD0043-1 mg, ATGD0043-10, ATGD0043-20, ATGD0043-50, ATGD0043-100, ATGD0043-250, ATGD0043-500, ATGD0043-1

Store the plasmid at -20C.

Lyophilized

Signal Transduction

600188

NP_001121.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001130.5

594bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.