AGS Lysate

AGS cells are derived from gastric adenocarcinoma of the stomach of a 54 year-old female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5) . AGS cells are used to suitable transfection host.

Human

WB

2 mg/mL (determined by BCA assay)

AGS, ATGL0030-10 µg, ATGL0030-20 µg, ATGL0030-50 µg, ATGL0030-100 µg, ATGL0030-250 µg, ATGL0030-500 µg, ATGL0030-1 mg, ATGL0030-10, ATGL0030-20, ATGL0030-50, ATGL0030-100, ATGL0030-250, ATGL0030-500, ATGL0030-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : AGS Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody