AK1 cDNA

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.

HTL-S-58j

9q34.1

Human

PATGen (puc19-derived cloning vector)

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGTGGGCC TGGCTCAGGG AAGGGCACCC AGTGTGAGAA GATCGTGCAG AAGTATGGCT ACACCCACCT CTCCACCGGG GACCTCCTGC GGTCCGAGGT CAGCTCAGGC TCGGCCAGGG GCAAGAAGCT GTCGGAAATC ATGGAGAAGG GGCAGCTGGT TCCACTGGAG ACAGTGTTGG ACATGCTCCG GGATGCCATG GTGGCCAAAG TCAATACTTC CAAAGGCTTC CTGATTGATG GCTACCCGCG GGAGGTGCAG CAAGGAGAAG AGTTTGAGCG ACGGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA GGCCCTGAGA CCATGACCCA GCGGCTCTTG AAACGTGGAG AGACCAGCGG GCGTGTGGAC GACAATGAGG AGACCATCAA AAAGCGGCTG GAGACCTATT ACAAGGCCAC AGAACCCGTC ATCGCCTTCT ATGAGAAACG TGGCATTGTG CGCAAGGTCA ACGCTGAGGG CTCCGTGGAC AGTGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTAA AGTAG

AK1, HTL-S-58j, AK1, ATGD0070-10 µg, ATGD0070-20 µg, ATGD0070-50 µg, ATGD0070-100 µg, ATGD0070-250 µg, ATGD0070-500 µg, ATGD0070-1 mg, ATGD0070-10, ATGD0070-20, ATGD0070-50, ATGD0070-100, ATGD0070-250, ATGD0070-500, ATGD0070-1

Store the plasmid at -20C.

Lyophilized

Metabolism

103000

NP_000467.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_000476.2

585bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.