AKR1D1 cDNA

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction.

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

7q32-q33

Human

PATGen (puc19-derived cloning vector)

ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC CCATTTCATG ATGAATACTG A

AKR1D1, 3o5bred, CBAS2, SRD5B1, AKR1D1, ATGD0024-10 µg, ATGD0024-20 µg, ATGD0024-50 µg, ATGD0024-100 µg, ATGD0024-250 µg, ATGD0024-500 µg, ATGD0024-1 mg, ATGD0024-10, ATGD0024-20, ATGD0024-50, ATGD0024-100, ATGD0024-250, ATGD0024-500, ATGD0024-1

Store the plasmid at -20C.

Lyophilized

Metabolism

604741

NP_005980.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_005989.3

981bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.