Akt (fosfo Ser473) Antilichaam

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

207

P31749

NP_001014431.1

Human, Mouse, Rat

Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

60 kDa

This antibody detects endogenous level of Akt only when phosphorylated at serine 473.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

AKT1

V-akt murine thymoma viral oncogene homolog 1

Homo sapiens

Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2) : 341-3. Min YH, et al. (2004) Cancer Res; 64 (15) : 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34) : 35510-7.

AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, NFKB3, C-AKT, PKB-alpha, RAC-PK-alpha

WB, IHC

62241013

Liquid