Akt (fosfo Thr308) Antilichaam

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

207

P31749

NP_001014431.1

Human, Mouse, Rat

Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

60 kDa

This antibody detects endogenous level of Akt only when phosphorylated at threonine 308.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

AKT1

V-akt murine thymoma viral oncogene homolog 1

Homo sapiens

Tremblay F, et al. (2005) Diabetes; 54 (9) : 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40) : 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6) : 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6) : 668-77.

AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, CFL, C-AKT, PKB-alpha, RAC-PK-alpha

WB, IHC, IF

62241013

Liquid