ALDOB cDNA

Fructose-bisphosphate aldolase B, also known as ALDOB, is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease

ALDB, ALDO2

9q21.3-q22.2

Human

PATGen (puc19-derived cloning vector)

ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC TGCTATACCT ACTAG

ALDOB, ALDB, ALDO2, ALDOB, ATGD0003-10 µg, ATGD0003-20 µg, ATGD0003-50 µg, ATGD0003-100 µg, ATGD0003-250 µg, ATGD0003-500 µg, ATGD0003-1 mg, ATGD0003-10, ATGD0003-20, ATGD0003-50, ATGD0003-100, ATGD0003-250, ATGD0003-500, ATGD0003-1

Store the plasmid at -20C.

Lyophilized

Metabolism

612724

NP_000026.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_000035.3

1095bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.