ALOX5AP Antilichaam/Arachidonaat 5-lip-zuurstof-activerende proteïne

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

P20292

Human

A synthetic peptide corresponding to a sequence in the middle region of human ALOX5AP was used as the immunogen for the ALOX5AP antibody.

WB, IHC, ICC, IF, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ALOX5AP antibody is available for research use only.

After reconstitution, the ALOX5AP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ALOX5AP antibody should be determined by the researcher.

ER, nuclear membrane

Immunofluorescent staining of ALOX5AP using anti-ALOX5AP antibody (green) . ALOX5AP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-ALOX5AP antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.