Ambra1 Antilichaam

Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

55626

Q9C0C7

Q9C0C7

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Autophagy

Ambra1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Horse (98%), Pig (97%)

Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Ambra1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

AMBRA1

Autophagy/beclin-1 regulator 1

Homo sapiens

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.

Liang XH, Jackson S, Seaman M, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature1999; 402:672-6.

Fimia GM, Stoykova A, Romagnoli A, et al. Ambra1 regulates autophagy and development of the nervous system. Nature2007; 447:1121-5.

Ambra1 Antibody: DCAF3, WDR94, KIAA1736, Activating molecule in BECN1-regulated autophagy protein 1

ELISA, ICC/IF, IHC-P, WB

166215833

Liquid