Amphireguline

Amphiregulin is an EGF-related growth factor that signals through the EGF/TGF-α receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Murine Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.

AR, AREG, Colorectum cell-derived growth factor (CRDGF)

11839

P31955

NP_033834.1

NM_009704.4

Mouse

Mouse

SVRVEQVIKP KKNKTEGEKS TEKPKRKKKG GKNGKGRRNK KKKNPCTAKF QNFCIHGECR YIENLEVVTC NCHQDYFGER CGEKSMKTHS EDDKDLSK

< 0.1 ng/µg of protein (< 1EU/µg)

≥ 95% by SDS-PAGE gel and HPLC analyses

Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

98

Lyophilized

11.3 kDa

E. coli