ANG-2 Aanbevolen eiwit

ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha helix rich "coiled coil” N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 - 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.

285

O15123

NP_001112359.1

Recombinant Proteins

CHO cells

Cancer, Cell Cycle

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Room Temp

The lyophilized ANG-2 recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted ANG-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing Yeast ANG-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Angiopoietin 2

Homo sapiens

ANG2, AGPT2, Angiopoietin-2, ANG-2

Human

169646749

Lyophilized