Anthrax Edema Factor Antilichaam

Anthrax Edema Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) . PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8) /anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. EF is a calmodulin and Ca++-dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system.

3361726

P40136

P40136

Bacteria

Unconjugated

Primary Antibodies

Infectious Disease

Anthrax Edema Factor Antibody is affinity chromatography purified via peptide column.

1 mg/mL

Anthrax Edema Factor Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Anthrax Edema Factor antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Anthrax Edema Factor antibody can be used for the detection of Anthrax PA protein in ELISA. It will detect 10 ng of free peptide at 1 μ g/mL.

Pxo1_122

Calmodulin-sensitive adenylate cyclase

Bacillus anthracis

Schwartz MN. Recognition and management of anthrax - an update. New Engl. J. Med. 2001; 345:1621-6.

Moayeri M and Leppla SH. The roles of anthrax toxin in pathogenesis. Curr. Opin. Microbiol. 2004; 7:19-24.

Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.

Scobie HM, Rainey GJ, Bradley KA, et al. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 2003; 100:5170-4.

Anthrax Edema Factor Antibody: Calmodulin-sensitive adenylate cyclase, ATP pyrophosphate-lyase, EF

ELISA

729244

Liquid