Anti-5 Lipoxygenase/ALOX5 Antibody Picoband® fluoro488 Vervoeging

ALOX5 (ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO (5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.

Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase;1.13.11.34; ALOX5; LOG5

ALOX5

240

P09917

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5, different from the related rat and mouse sequences by two amino acids.

Flow Cytometry

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Energy Metabolism, Energy Transfer Pathways, Heart Disease, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Immunogen affinity purified.

Liquid

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. .

1. Aliberti, J., Serhan, C., Sher, A. Parasite-induced lipoxin A4 is an endogenous regulator of IL-12 production and immunopathology in Toxoplasma gondii infection. J. Exp. Med. 196: 1253-1262, 2002. 2. Bafica, A., Scanga, C. A., Serhan, C., Machado, F., White, S., Sher, A., Aliberti, J. Host control of Mycobacterium tuberculosis is regulated by 5-lipoxygenase-dependent lipoxin production. J. Clin. Invest. 115: 1601-1606, 2005. 3. Funk CD, Hoshiko S, Matsumoto T, Rdmark O, Samuelsson B (April 1989) . Characterization of the human 5-lipoxygenase gene. Proc. Natl. Acad. Sci. U.S.A. 86 (8) : 2587–91.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

77983 MW

No cross reactivity with other proteins.

6

Arachidonate 5-lipoxygenase

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Belongs to the lipoxygenase family.

Arachidonate 5-lipoxygenase (5-LO/5-lipoxygenase)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.