Anti-A-Raf (N-terminal) Antilichaam

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3) . The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259) /B-Raf (S365) ) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and Ser-471. The latter site is phosphorylated after EGF stimulation and may be important for MEK interaction in both C-Raf and A-Raf. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity.

Our A-Raf (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat A-Raf (N-terminal region) and is protein g purified. It is great for use in ELISA, WB.

Our A-Raf (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat A-Raf (N-terminal region) and is Protein G purified. It is great for use in WB.

Araf

ARAF

P10398

Human, Mouse, and Rat

Clone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf.

A-Raf (N-terminal region)

M289

Unconjugated

ELISA, WB

Apoptosis, Cell Signaling, Kinase Signaling, Oncoproteins

Protein G Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

68

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein G purified culture supernatant.

1:1000

This antibody detects a 68 kDa* protein corresponding to the molecular mass of A-Raf on SDS-PAGE immunoblots of human SKN-SH and Jurkat cells.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2274390