Anti-α-Actinine 4 (N-terminaal) Antilichaam

α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through anti-parallel homodimers of the rod domains. Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. α-Actinin 1 is phosphorylated at Tyr-12 by FAK, while α-actinin 4 can be phosphorylated at Tyr-4 and Tyr-31 after EGF treatment. Tyr-4 and Tyr-31 phosphorylation inhibit actin binding and reduces actin-filament driven multi-nucleation in rat kidney cells. Thus, phosphorylation in α-actinins may be important for regulating actin binding and actin cytoskeletal remodeling.

Our α-Actinin 4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat α-Actinin 4 and is antigen affinity purified. It is great for use in ELISA, WB.

Our α-Actinin 4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat α-Actinin 4 and is antigen affinity purified. It is great for use in WB.

A-actinin 4, actinin alpha4

ACTN4

O43707

Human, Mouse, and Rat

α-Actinin 4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human α-actinin 4. This sequence is well conserved in rat and mouse α-actinin 4, but is not conserved in other α-actinins.

α-Actinin 4

Unconjugated

ELISA, WB

Cytoskeletal Regulation, Extracellular Matrix Proteins, Kidney Function, Vesicle Trafficking/Fusion

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

100

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

The antibody detects a 100 kDa* protein corresponding to the molecular mass of α-actinin 4 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_10837643