Anti-Adalimumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Adalimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Adalimumab will be captured by immobilized Adalimumab. After washing away any unbound substances, a biotin-labeled Adalimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Adalimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Adalimumab

ABP 501, D2E7, Adalimumab, CAS: 331731-18-1

Colorimetric

Quantitative

Plasma, Serum

156.25 - 10,000 ng/mL

80-120%

83.1 ng/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.