Anti-adenylaatcyclase type III (ACIII) Antilichaam

Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons, and our antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. Adenylate cyclase type III is a large complex molecule of, in the human, 1145 amino acids with a deduced molecular weight of 129 kDa. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160 kDa in cortex and about 200 kDa in olfactory epithelium. The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.

Our Anti-Adenylate cyclase type III (ACIII) rabbit polyclonal primary antibody detects human (predicted), mouse, other mammals (predicted), and rat Adenylate cyclase type III (ACIII), and is affinity-purified. It is validated for use in ICC, IHC, WB.

Our Anti-Adenylate cyclase type III (ACIII) rabbit polyclonal primary antibody detects human (predicted), mouse, other mammals (predicted), and rat Adenylate cyclase type III (ACIII), and is affinity-purified. It is validated for use in ICC, IHC, WB.

P21932

Human (Predicted), Mouse, Other Mammals (Predicted), and Rat

20 amino acid peptide identical to the C-terminus of rat ACIII (amino acids PAAFPNGSSVTLPHQVVDNP)

Adenylate cyclase type III (ACIII)

Unconjugated

ICC, IHC, WB

Affinity-purified

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

200 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.

1:5000-1:10000

1:1000-1:2000

The antibody stains a band at about 200 kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160 kDa. It has also been used successfully for immunocytochemistry on mixed neuron/glia cultures. The antibody has been directly tested for reactivity in rat. It is expected that it will work on human due to homology with the immunogen and possibly other mammal tissues (human, horse, cow, pig, chicken, mouse) .

1:5000-1:10000

Rabbit

Lyophilized

IgG

Rat