Anti-Alkaline Fosfatase/ALPL Antibody Picoband®

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone) . Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts , the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) . ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP;3.1.3.1 ; Alkaline phosphatase liver/bone/kidney isozyme; ALPL

ALPL

249

P05186

Human

No cross-reactivity with other proteins.

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related rat and mouse sequences by two amino acids.

Widely expressed, with highest expression in brain. .

IHC, WB

Cancer, Cell Biology, Cell Cycle, Kinases/Phosphatases, Tags & Cell Markers, Tumor Biomarkers

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

This isozyme may play a role in skeletal mineralization.

1. Fedde, K. N.; Whyte, M. P. : Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. 2. Weiss, M. J.; Cole, D. E. C.; Ray, K.; Whyte, M. P.; Lafferty, M. A.; Mulivor, R. A.; Harris, H. : A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc. Nat. Acad. Sci. 85: 7666-7669, 1988.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1004

57305 MW

80 kDa

No cross reactivity with other proteins.

Alkaline phosphatase, tissue-nonspecific isozyme

Cell membrane ; Lipid-anchor, GPI-anchor .

Contains 12 fibronectin type-I domains.

Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP/TNSALP)

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.