Anti-Alkaline Fosfatase/ALPL Antibody Picoband® fluorazo647 Vervoeging

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone) . Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) . ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP;3.1.3.1 ; Alkaline phosphatase liver/bone/kidney isozyme; ALPL

ALPL

249

P05186

Human

No cross-reactivity with other proteins.

A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase, different from the related mouse and rat sequences by three amino acids.

Widely expressed, with highest expression in brain. .

Flow Cytometry

Cell Biology, Cell Cycle, Kinases/Phosphatases, Tags & Cell Markers, Tumor Biomarkers

Immunogen affinity purified.

Liquid

This isozyme may play a role in skeletal mineralization.

1. Fedde, K. N.; Whyte, M. P. : Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. 2. Weiss, M. J.; Cole, D. E. C.; Ray, K.; Whyte, M. P.; Lafferty, M. A.; Mulivor, R. A.; Harris, H. : A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc. Nat. Acad. Sci. 85: 7666-7669, 1988.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

57305 MW

No cross reactivity with other proteins.

6

Alkaline phosphatase, tissue-nonspecific isozyme

Cell membrane ; Lipid-anchor, GPI-anchor .

Contains 12 fibronectin type-I domains.

Alkaline phosphatase, tissue-nonspecific isozyme

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.