Anti-Angiopoietin 2/ANGPT2 Antibody Picoband® fluoro488 Vervoeging

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Angiopoietin-2; ANG-2; ANGPT2

ANGPT2

285

O15123

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin 2.

Widely expressed, with highest expression in brain. .

Flow Cytometry

Angiogenesis, Cardiovascular, Growth Factors

Immunogen affinity purified.

Liquid

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

1. Kim, I.; Kim, J.-H.; Ryu, Y. S.; Jung, S. H.; Nah, J. J.; Koh, G. Y. : Characterization and expression of a novel alternatively spliced human angiopoietin-2. J. Biol. Chem. 275: 18550-18556, 2000. 2. Maisonpierre, P. C.; Suri, C.; Jones, P. F.; Bartunkova, S.; Wiegand, S. J.; Radziejewski, C.; Compton, D.; McClain, J.; Aldrich, T. H.; Papadopoulos, N.; Daly, T. J.; Davis, S.; Sato, T. N.; Yancopoulos, G. D. : Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 277: 55-60, 1997. 3. Ward, E. G.; Grosios, K.; Markham, A. F.; Jones, P. F. : Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2. Cytogenet. Cell Genet. 94: 147-154, 2001.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

56919 MW

No cross reactivity with other proteins.

6

Angiopoietin-2

Secreted.

Contains 1 fibrinogen C-terminal domain.

Angiopoietin-2 (ANG-2)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.