Anti-Apoptosis repressor met CARD/NOL3 Antibody Picoband® fluoro488 Vervoeging

NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Nucleolar protein 3 ; Apoptosis repressor with CARD ; Muscle-enriched cytoplasmic protein ; Myp ; Nucleolar protein of 30 kDa ; Nop30 ; NOL3 ; ARC , NOP

NOL3

8996

O60936

Human, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

Flow Cytometry

Apoptosis, Caspases, Cell Biology, Intracellular

Immunogen affinity purified.

Liquid

Isoform 1: May be involved in RNA splicing. .

1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: 213-221, 2004. 2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998. 3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. Chem. 274: 10951-10962, 1999.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

23 kDa

No cross reactivity with other proteins.

6

Nucleolar protein 3

Isoform 1: Nucleus, nucleolus . The SR-rich C-terminus mediates nuclear localization. .

Contains 1 CARD domain.

Nucleolar protein 3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.