Anti-Argonaute 2 Antilichaam

Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

Our Argonaute 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Argonaute 2 and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our Argonaute 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Argonaute 2 and is antigen affinity purified. It is great for use in WB, ICC.

Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer

AGO2

Q9UKV8

Chicken, Human, Mouse, Rat, and Xenopus

Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids from the central region of human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.

Argonaute 2

Unconjugated

ELISA, WB, ICC

DNA Damage and Repair, Epigenetics

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

97

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of Ago2 on SDS-PAGE immunoblots of human A431 cells and mouse recombinant Ago2 full length protein.

1:100

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068366