Anti-Arp3 (C-terminaal gebied) Antilichaam

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.

Our Arp3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Arp3 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IHC.

Our Arp3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Arp3 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

ACTR3, Arp3

ACTR3

P61158

Chicken, Human, Mouse, Rat, and Xenopus

Arp3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins.

Arp3 (C-terminal region)

Unconjugated

ELISA, WB, ICC, IHC

Cytoskeletal Regulation, Extracellular Matrix Proteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

47

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:100

1:1000

The antibody detects a 47 kDa* doublet corresponding to the molecular mass of Arp3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.

1:250

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2734127