Anti-Arpc1b/p41-Arc (Thr-21), Fosfospecifiek antilichaam

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21

Our Arpc1b/p41-Arc (Thr-21) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus Arpc1b/p41-Arc (Thr-21) and is antigen affinity purified. It is great for use in ELISA, WB.

Our Arpc1b/p41-Arc (Thr-21) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Arpc1b/p41-Arc (Thr-21) and is antigen affinity purified. It is great for use in WB.

Arc41, p41, Arpc1b

ARPC1B

O15143

Thr-21

Chicken, Human, Mouse, Rat, and Xenopus

Phospho-Arpc1b/p41-Arc (Thr-21) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a.

Arpc1b/p41-Arc (Thr-21)

Unconjugated

ELISA, WB

Cytoskeletal Regulation, Extracellular Matrix Proteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

41

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and mouse C2C12 treated with calyculin A. This reactivity is removed with lambda phosphatase treatment.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068365