Anti-ATG4B Antibody Picoband® fluoro594 Vervoeging

Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase; GK

ATG4B

23192

Q9Y4P1

Human, Rat

No cross-reactivity with other proteins.

E.coli-derived human ATG4B recombinant protein (Position: M1-L392) .

Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.

Flow Cytometry

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Immunogen affinity purified.

Liquid

Key enzyme in the regulation of glycerol uptake and metabolism.

1. Agrotis, A., Pengo, N., Burden, J. J., Ketteler, R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15: 976-997, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/8/2019. 3. Hemelaar, J., Lelyveld, V. S., Kessler, B. M., Ploegh, H. L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J. Biol. Chem. 278: 51841-51850, 2003.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Glycerol kinase

Mitochondrion outer membrane; In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.