Anti-ATP6V1A Antibody Picoband®

V-type proton ATPase catalytic subunit A is an enzyme that in humans is encoded by the ATP6V1A gene. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.

Boster Bio Anti-ATP6V1A Antibody Picoband® catalog # A10401-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

ATP6V1A

523

P38606

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human ATP6V1A recombinant protein (Position: R129-D617) .

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Hematopoietic Progenitors, Lymphoid, Stem Cells, T Lymphocytic Lineage

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

1. Fassio, A., Esposito, A., Kato, M., Saitsu, H., Mei, D., Marini, C., Conti, V., Nakashima, M., Okamoto, N., Olmez Turker, A., Albuz, B., Semerci Gunduz, C. N., and 14 others. De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy. Brain 141: 1703-1718, 2018. 2. Gross, M. B. Personal Communication. Baltimore, Md. 1/3/2019. 3. Ramirez, C., Hauser, A. D., Vucic, E. A., Bar-Sagi, D. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature 576: 477-481, 2019.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A10401-2

41409 MW

70 kDa

6

Pre T cell antigen receptor alpha

Membrane; Single-pass type I membrane protein.

Protein NDRG3

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.