Anti-ATP6V1A Antibody Picoband® fluoro488 Vervoeging

V-type proton ATPase catalytic subunit A is an enzyme that in humans is encoded by the ATP6V1A gene. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.

Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

ATP6V1A

523

P38606

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human ATP6V1A recombinant protein (Position: R129-D617) .

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Flow Cytometry

Hematopoietic Progenitors, Lymphoid, Stem Cells, T Lymphocytic Lineage

Immunogen affinity purified.

Liquid

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

1. Fassio, A., Esposito, A., Kato, M., Saitsu, H., Mei, D., Marini, C., Conti, V., Nakashima, M., Okamoto, N., Olmez Turker, A., Albuz, B., Semerci Gunduz, C. N., and 14 others. De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy. Brain 141: 1703-1718, 2018. 2. Gross, M. B. Personal Communication. Baltimore, Md. 1/3/2019. 3. Ramirez, C., Hauser, A. D., Vucic, E. A., Bar-Sagi, D. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature 576: 477-481, 2019.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

41409 MW

6

Pre T cell antigen receptor alpha

Membrane; Single-pass type I membrane protein.

Protein NDRG3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.