Anti-AUP1 Antibody Picoband® 31.0550 Vervoeging

Ancient ubiquitous protein 1 is a protein that in humans is encoded by the AUP1 gene. The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR) . Alternative splicing results in multiple transcript variants.

DnaJ homolog subfamily A member 2; Cell cycle progression restoration gene 3 protein; Dnj3; Dj3; HIRA-interacting protein 4; Renal carcinoma antigen NY-REN-14; DNAJA2; CPR3; HIRIP4

AUP1

550

Q9Y679

Human

No cross-reactivity with other proteins.

E.coli-derived human AUP1 recombinant protein (Position: A77-R399) .

Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Flow Cytometry

Organelles, Subcellular Markers, Tags & Cell Markers

Immunogen affinity purified.

Liquid

Co-chaperone of Hsc70. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B.

1. Jang, W., Weber, J. S., Bashir, R., Bushby, K., Meisler, M. H. Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13. Genomics 36: 366-368, 1996. 2. Mueller, B., Klemm, E. J., Spooner, E., Claessen, J. H., Ploegh, H. L. SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins. Proc. Nat. Acad. Sci. 105: 12325-12330, 2008. 3. Spandl, J., Lohmann, D., Kuerschner, L., Moessinger, C., Thiele, C. Ancient ubiquitous protein 1 (AUP1) localizes to lipid droplets and binds the E2 ubiquitin conjugase G2 (Ube2g2) via its G2 binding region. J. Biol. Chem. 286: 5599-5606, 2011.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

DnaJ heat shock protein family (Hsp40) member A2

Membrane. Lipid-anchor.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.