Anti-Autofagy eiwit 5 (ATG5) Antilichaam

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.

Our Anti-Autophagy protein 5 (ATG5) rabbit polyclonal primary antibody detects human Autophagy protein 5 (ATG5), and is igg. It is validated for use in IHC-Frozen, WB.

Our Anti-Autophagy protein 5 (ATG5) rabbit polyclonal primary antibody detects human Autophagy protein 5 (ATG5), and is IgG. It is validated for use in IHC-Frozen, WB.

Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP

Q9H1Y0

Human

A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

Autophagy protein 5 (ATG5)

Unconjugated

IHC-Frozen, WB

IgG

Lyophilized from a solution containing PBS pH 7.4

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 500 μL of sterile-filtered, ultra pure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Protein G purified IgG

1:200-1:1000

1:200-1:1000

IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.

1:200-1:1000

Rabbit

Lyophilized

IgG

Human