Anti-Axin 2/AXIN2 Antibody Picoband® fluoro550 Vervoeging

Axin-2 also known as axin-like protein (Axil) or axis inhibition protein 2 (AXIN2) or conductin is a protein that in humans is encoded by the AXIN2 gene. The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Ubiquitin-like-conjugating enzyme ATG3;6.3.2.-; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3, APG3L

AXIN2

8313

Q9Y2T1

Human

No cross-reactivity with other proteins.

E.coli-derived human Axin 2/AXIN2 recombinant protein (Position: D614-D843) .

Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. .

Flow Cytometry

Cell Type Marker, Hematopoietic Progenitors, Neural Stem Cells, Neuroscience, Stem Cells, Surface Molecules

Immunogen affinity purified.

Liquid

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) . The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE) . This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt) . Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. .

1. Callahan, N., Modesto, A., Meira, R., Seymen, F., Patir, A., Vieira, A. R. Axis inhibition protein 2 (AXIN2) polymorphisms and tooth agenesis. Arch. Oral Biol. 54: 45-49, 2009. 2. Dong, X., Seelan, R. S., Qian, C., Mai, M., Liu, W. Genomic structure, chromosome mapping and expression analysis of the human AXIN2 gene. Cytogenet. Cell Genet. 93: 26-28, 2001. 3. Lammi, L., Arte, S., Somer, M., Jarvinen, H., Lahermo, P., Thesleff, I., Pirinen, S., Nieminen, P. Mutations in AXIN2 cause familial tooth agenesis and predispose to colorectal cancer. Am. J. Hum. Genet. 74: 1043-1050, 2004.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

35864 MW

6

Ubiquitin-like-conjugating enzyme ATG3

Cytoplasm .

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.