Anti-BAF57/SMARCE1 Antibody Picoband®

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 is a protein that in humans is encoded by the SMARCE1 gene. The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Boster Bio Anti-BAF57/SMARCE1 Antibody Picoband® catalog # A03739-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Histone-lysine N-methyltransferase SETD1A; Lysine N-methyltransferase 2F; SET domain-containing protein 1A; Hset1a; Set1/Ash2 histone methyltransferase complex subunit SET1; SETD1A; KIAA0339; KMT2F; SET1; SET1A

SMARCE1

6605

Q969G3

Human, Monkey, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human BAF57/SMARCE1 recombinant protein (Position: Q18-A293) .

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Calcium Binding Proteins, Calcium Signaling, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Signaling Pathway

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

1. Chi, T. H., Wan, M., Zhao, K., Taniuchi, I., Chen, L., Littman, D. R., Crabtree, G. R. Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes. Nature 418: 195-199, 2002. 2. Smith, M. J., O'Sullivan, J., Bhaskar, S. S., Hadfield, K. D., Poke, G., Caird, J., Sharif, S., Eccles, D., Fitzpatrick, D., Rawluk, D., du Plessis, D., Newman, W. G., Evans, D. G. Loss-of-function mutations in SMARCE1 cause an inherited disorder of multiple spinal meningiomas. Nature Genet. 45: 295-298, 2013. 3. Tsurusaki, Y., Okamoto, N., Ohashi, H., Kosho, T., Imai, Y., Hibi-Ko, Y., Kaname, T., Naritomi, K., Kawame, H., Wakui, K., Fukushima, Y., Homma, T., and 19 others. Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome. Nature Genet. 44: 376-378, 2012.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A03739-2

54 kDa

6

SET domain containing 1A, histone lysine methyltransferase

Nucleus speckle. Chromosome.

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.