Anti-BAK/BAK1 Antibody Picoband®

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene.The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons.By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6.This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Boster Bio Anti-BAK/BAK1 Antibody catalog # PA1437. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK, BCL2L7, CDN1

BAK1

578

Q16611

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human BAK1, different from the related mouse and rat sequences by one amino acid.

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

IF, IHC, ICC, WB

Signal Transduction, Signaling Pathway

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. .

1. Chittenden, T., Harrington, E. A., O'Connor, R., Flemington, C., Lutz, R. J., Evan, G. I., Guild, B. C.Induction of apoptosis by the Bcl-2 homologue Bak.Nature 374: 733-736, 1995. 2. Herberg, J. A., Phillips, S., Beck, S., Jones, T., Sheer, D., Wu, J. J., Prochazka, V., Barr, P. J., Kiefer, M. C., Trowsdale, J.Genomic structure and domain organisation of the human Bak gene.Gene 211: 87-94, 1998. 3. Kiefer, M. C., Brauer, M. J., Powers, V. C., Wu, J. J., Umansky, S. R., Tomei, L. D., Barr, P. J.Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.Nature 374: 736-739, 1995.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1437

23409 MW

23 kDa

No cross reactivity with other proteins.

Bcl-2 homologous antagonist/killer

Mitochondrion membrane ; Single-pass membrane protein .

Belongs to the Bcl-2 family.

Bcl-2 homologous antagonist/killer

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.