Anti-BAK/BAK1 Antibody Picoband® fluoro594 Vervoeging

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene.The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons.By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6.This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK, BCL2L7, CDN1

BAK1

578

Q16611

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human BAK1, different from the related mouse and rat sequences by one amino acid.

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Flow Cytometry

Signal Transduction, Signaling Pathway

Immunogen affinity purified.

Liquid

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. .

1. Chittenden, T., Harrington, E. A., O'Connor, R., Flemington, C., Lutz, R. J., Evan, G. I., Guild, B. C.Induction of apoptosis by the Bcl-2 homologue Bak.Nature 374: 733-736, 1995. 2. Herberg, J. A., Phillips, S., Beck, S., Jones, T., Sheer, D., Wu, J. J., Prochazka, V., Barr, P. J., Kiefer, M. C., Trowsdale, J.Genomic structure and domain organisation of the human Bak gene.Gene 211: 87-94, 1998. 3. Kiefer, M. C., Brauer, M. J., Powers, V. C., Wu, J. J., Umansky, S. R., Tomei, L. D., Barr, P. J.Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.Nature 374: 736-739, 1995.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

23409 MW

No cross reactivity with other proteins.

6

Bcl-2 homologous antagonist/killer

Mitochondrion membrane ; Single-pass membrane protein .

Belongs to the Bcl-2 family.

Bcl-2 homologous antagonist/killer

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.