Anti-BAP1 Antibody Picoband® 31.0550 Vervoeging

BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1-associated protein 1; Cerebral protein 6; BAP1; KIAA0272; hucep-6

BAP1

8314

Q92560

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human BAP1 recombinant protein (Position: E7-Q702) .

Highly expressed in testis, placenta and ovary. Expressed in breast.

Flow Cytometry

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Deubiquitination, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers

Immunogen affinity purified.

Liquid

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) . Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950) . Acts as a tumor suppressor.

1. Jensen, D. E.; Proctor, M.; Marquis, S. T.; Gardner, H. P.; Ha, S. I.; Chodosh, L. A.; Ishov, A. M.; Tommerup, N.; Vissing, H.; Sekido, Y.; Minna, J.; Borodovsky, A.; Schultz, D. C.; Wilkinson, K. D.; Maul, G. G.; Barlev, N.; Berger, S. L.; Prendergast, G. C.; Rauscher, F. J., III : BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene 16: 1097-1112, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

80 kDa

6

BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)

Cytoplasm.

Beta-nerve growth factor

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.