Anti-BCRP/ABCG2 Antibody Picoband®

ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX,   is a  protein that in humans is encoded by the ABCG2  gene. The  ABCG2  gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes.  The  ABCG2  protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with  ABCG2  expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001)   concluded that  ABCG2  is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2  transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food.  ABCG2  is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1869. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD338; ABCG2; ABCP, BCRP, BCRP1, MXR

ABCG2

9429

Q9UNQ0

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human BCRP/ABCG2.

Highly expressed in placenta. Low expression in small intestine, liver and colon. .

WB

ATPases, Cancer, Cell Type Markers, Drug Metabolism, Hematopoietic Progenitors, Metabolic Signaling Pathways, Metabolism, Neural Stem Cells, Pathways and Processes, Plasma Membrane, Signal Transduction, Stem Cells, Surface Molecules, Tags & Cell Markers, Tumor Associated

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. .

1. Jonker, J. W., Merino, G., Musters, S., van Herwaarden, A. E., Bolscher, E., Wagenaar, E., Mesman, E., Dale, T. C., Schinkel, A. H. The breast cancer resistance protein BCRP (ABCG2) concentrates drugs and carcinogenic xenotoxins into milk. Nature Med. 11: 127-129, 2005. 2. Matsuo, H., Takada, T., Ichida, K., Nakamura, T., Nakayama, A., Ikebuchi, Y., Ito, K., Kusanagi, Y., Chiba, T., Tadokoro, S., Takada, Y., Oikawa, Y., and 22 others. Common defects of ABCG2, a high-capacity urate exporter, cause gout: a function-based genetic analysis in a Japanese population.Sci. Transl. Med. 1: 5ra11, 2009. 3. Ozvegy, C., Litman, T., Szakacs, G., Nagy, Z., Bates, S., Varadi, A., Sarkadi, B. Functional characterization of the human multidrug transporter, ABCG2, expressed in insect cells.Biochem. Biophys. Res. Commun. 285: 111-117, 2001.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1869

72314 MW

75 kDa

No cross reactivity with other proteins.

ATP-binding cassette sub-family G member 2

Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

ATP-binding cassette sub-family G member 2

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.